Applying Ligand-observe NMR Spectroscopy to Enable Fragment-based Lead Discovery
Date: Nov 15, 2011
Time: 11:00 – 11:50 AM PST
Speaker: Darren Begley, Ph.D.
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Nuclear magnetic resonance (NMR) spectroscopy can provide fast, powerful, and versatile techniques for atomic-level resolution studies of protein-ligand interactions. NMR methods are well-suited to monitor and characterize small molecule-macromolecule interactions for weak-binding compounds, such as those used in fragment-based screening. Data acquired by NMR for protein-ligand interactions in solution can be particularly valuable in cases where structural information on a macromolecular complex is either difficult or impossible to obtain.
In this free 45 minute webinar, several case studies will be used to illustrate information obtainable by NMR, and highlight some of the advantages in using NMR-based, hit-to-lead strategies in comparison to other biophysical methods. We’ll discuss some of the practical aspects of ligand-observe NMR methods for fragment-based screening and lead discovery, including:
• NMR sample preparation and library assembly
• Data acquisition and NMR spectra interpretation for primary fragment-based screens
• Follow-up experiments for hit characterization