MTPP Focus on Molecular Biology: Implementing a Parallel Processing Approach to Construct Design and Expression Testing
Date: Jul 17, 2012
Time: 11:00 – 11:50 AM PDT
Speaker: Amy Raymond, Ph.D., Group Leader, Emerald BioStructures
Technological advances in the forms of new tools and procedures have revolutionized access to protein structure determination by X-ray crystallography by dramatically lowering time and cost. These tools and procedures enable structural biologists to rapidly advance protein crystallography projects by allowing the researcher to select multiple target constructs and prosecute them in parallel, thus compressing the gene-to-protein part of the gene-to-structure pathway. Learn how this can be accomplished through High Throughput methodologies for parallel cloning. By putting your gene into a variety contexts followed by screening the array of constructs for expression and solubility, you will gain critical insights as to which constructs will provide the highest return on investment at the preparative scale. In this free 40 minute webinar, several case studies will be used to illustrate how concepts from the Multi-Target Parallel Processing™ platform (MTPP) at Emerald BioStructures can be applied in moderate sized laboratories. Topics included will be:
- Structure-guided construct design
- High Throughput/Parallel cloning strategies
- Use of structure-specific proteases and fusion partners as screening tools