Multi-Target Parallel Processing or, How to Turn a Dozen New Targets into a SBDD Pipeline in Less Than a Year
Date: Mar 20, 2012
Time: 11:00 – 11:50 AM PDT
Speaker: Bart Staker, PhD, Director of Multi-Target Services, Emerald BioStructures
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The advances of structural genomics technology have revolutionized the time and cost of protein structure determination by X-ray crystallography. New tools and procedures are available today which enable structural biologists to rapidly advance protein crystallography projects involving sets of six or more targets. The essence of this success can be distilled into the concept of selecting multiple target constructs and prosecuting them in parallel. In this free 45 minute webinar, several case studies will be used to illustrate how concepts from the multi-target parallel processing platform at Emerald BioStructures can be applied in moderate sized laboratories. We will discuss the structure determination of multiple targets from the methylerythritol phosphate (MEP) pathway from Pseudomonas aerurginosa and Klebsiella pneumonia. Additionally we will discuss how single target poor crystallization hits can be rapidly improved by creating multiple derivative constructs. Topics included will be:
- Multi-target selection for protein structure based methods
- Expression system selection, parallel screening and purification
- Optimizing crystallization and expression properties through construct design